| Issue |
Photoniques
Number 134, 2025
Optics for Life Sciences
|
|
|---|---|---|
| Page(s) | 36 - 41 | |
| Section | Focus: optics for life sciences | |
| DOI | https://doi.org/10.1051/photon/202513436 | |
| Published online | 28 November 2025 | |
Smart illumination for 3D-imaging of biological tissues
Aix Marseille Univ, CNRS, Centrale Med, Institut Fresnel, 13013 Marseille, France
Three dimensional imaging of living tissues with widefield fluorescence optical microscopy is limited by out-of-focus blur, light dose, and acquisition speed. We discuss recent camera-based strategies that use light more intelligently to improve resolution, sectioning and reduce toxicity when imaging thick biological structures.
© EDP Sciences, 2025
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